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ELISA. Asaia strains expressing the fusion partner constructs were grown overnight. Cultures were streaked on mannitol plates and grown at 30°C for 48 h. Colonies were collected by flooding the plates with 1 mL of minimal media, gently scraping the cells from the plate, and collecting the liquid into centrifuge tubes.Asaia sp. was abundant in the mosquito body, with bacterial counts of up to 9.8 105 colony forming units (CFU) per female and 9.8 104 CFU per male individuals. To evaluate the relative abundance of Asaia sp. in different organs of An. stephensi, we measured Asaia to total bacteria 16S rRNA gene Asaia to total bacteria copy ratio (ABR) with ...Asaia prunellae JCM 25354 Asaia siamensis Asaia siamensis NRIC 0323 Asaia spathodeae Asaia spathodeae NBRC 105894 unclassified Asaia Asaia sp. Asaia sp. 107(2012) Asaia sp. 12F Asaia sp. 251001 Asaia sp. 85 Asaia sp. 8F Asaia sp. AA 1.5 Ab Asaia sp. AA 1.6 Ab Asaia sp. AA 3.7 Aa Asaia sp. 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